Planned Parenthood announced Thursday its center in Muncie will close Friday.
Planned Parenthood officials say the decisions are unlikely to have an effect on the smaller-than-average number of clinics currently operating in Indiana.
New changes to state law require an ultrasound at least 18 hours before an abortion.
Planned Parenthood and the ACLU are celebrating the judge's decision, but anti-abortion groups are urging the state to appeal the ruling.
A judge granted the preliminary injunction sought by Planned Parenthood of Indiana and Kentucky. The law was to set to take effect Friday.
Planned Parenthood and the American Civil Liberties Union of Indiana sued the state in April.
The federal judge indicated a ruling will come before the law takes effect July 1st.
Planned Parenthood of Indiana and Kentucky sued the state in April, calling the law unconstitutional and seeking to prevent it from taking effect July 1.
Planned Parenthood and the ACLU are suing the state over a bill passed this year, which bans abortions based on race, sex, and disability.
Known as House Enrolled Act 1337, the law bans abortions because of a fetus's gender, race or genetic disability, such as Down syndrome.