The ruling, which LGBT rights organizations are hailing as a "game changer," stems from a case originating in South Bend, Indiana.
The county's anti-discrimination ordinance will now include sexual orientation and gender identity.
The Trump administration is leaving the issue up to states to decide. Indiana school officials are divided on the issue.
The cities of Indianapolis, Bloomington, Carmel and Columbus were appealing a decision by a Hamilton Superior Court judge.
The state is appealing a ruling that allows both lesbian parents to be listed on a birth certificate.
Indiana Youth Group said Tuesday its 15,000 square feet of office space on Indianapolis' north side is about five times the size of its former location.
The LGBTQ CommUnity Center emphasizes "unity" and will serve as a safe space for those in need.
Anti-discrimination legislation for lesbian, gay, bisexual and transgender Hoosiers couldn’t make it out of committee last session.
The lawsuit alleges ordinances passed in several Indiana cities violate RFRA.
The federal judge says denying both same-sex spouses the right to be on a birth certificate leaves the child having only one legal parent.