Environmentalists have fought the 47-mile tollway plan, arguing it would threaten the Midewin National Tallgrass Prairie south of Chicago.
Indiana officials are asking motorists to be more watchful for deer on the state's roadways as fall weather and mating season approach.
More than 60 rural communities around Indiana are sharing nearly $80 million in federal funding for road and bridge improvements and other projects.
INDOT calls the employee's email "an inappropriate use of INDOT resources."
The Bloomington company halted work on the project earlier this month because the private developer owed Crider and Crider more than $2.3 million.
Bloomington's mayor says at least one subcontractor walked off the job Friday because of an ongoing pay dispute.
The I-65 project between Greenwood and Franklin has caused delays since April 2015.
INDOT is hosting a series of meetings for Indiana Farm Bureau members to discuss road and bridge funding.
The Hoosier State Line runs from Indianapolis to Chicago.
Police say drivers should avoid the southbound lanes of State Road 37 between Martinsville and Bloomington between noon and 3:30 p.m.