The Indiana High School Athletic Association Board of Directors has approved the new rule.
The Animal Board of Health began requiring a blood test for a parasitic disease a couple of years ago after several outbreaks around the country.
Maurice Creek said he will play his senior year at another school.
The 63rd Little 500 men's race took place this Saturday at the Bill Armstrong Stadium at Indiana University.
For the second year in a row, the Delta Gamma cycling team won the championship in the women's Little 500 bicycle race.
The Colts have the No. 24 pick in this year's NFL draft.
Law enforcement plans to work during the late night and early morning hours of the Little 500 weekend, with help from state troopers and excise police.
The sophomore guard is the third Hoosier to leave the team this week.
Cody Zeller will follow his teammate Victor Oladipo to the NBA draft.
The junior guard will forego a final year of college eligibility for a chance to be a pick in the NBA Draft Lottery later this year.
The IU Hoosiers ended their season Thursday after losing to Syracuse in the Sweet Sixteen matchup.